図5. カテコールアミン(2)

Column: ReproSil-Pur C18-AQ, 120Å, 5µm, 250×4mm
Sample: Catecholamines-2
1 = Noradrenaline
2 = Adrenaline
3 = D/L-3,4-Dihydroxyphenyl acetic acid (DOPAC)
4 = 3,4-Dihydroxyphenylalanine
5 = Dopamine
6 = Tyrosine
7 = D/L-Methoxy-4-hydroxymandelic acid
8 = Phenylalanine
9 = 4-Hydroxy-3-methoxyphenylglycol
10 = 5-Hydroxyindole-3-acetic acid
11 = Vanillic acid
12 = Homovanillic acid
Injection: 50-200µl/ml
Eluent A: 100mM Na-Phosphate, pH 3.0
Eluent B: ACN
Gradient: 1% B in 20min then 1-15% B in 15min
Flow rate: 1.0ml/min
Detection: UV210nm