図12. オリゴヌクレオチド

Column: ReproSil-Pur C18-AQ, 120Å, 5µm, 150×8mm
Guard column: ReproSil-Pur C18 -AQ, 120Å, 5µm, 30×8mm
Sample: Oligonucleotides
Eluent A: 15% 0.1M TEAA : 85% ACN
Eluent B: 30% 0.1M TEAA : 70% ACN
Gradient: 0 -100% B in 13min
Flow rate: 4.0ml/min
Detection: UV260nm