Experimental Conditions

Columns:
1.7µm Evosphere C18/AR 100x2.1mm
1.7µm Evosphere RP18-Amide 100x2.1mm

Mobile phase
A: 95:5 Water : MeOH + 2mM ammonium acetate
B: Methanol + 2mM ammonium acetate

Gradient:
Time
0
1
6
13
14
17
18

%B
0
20
45
80
95
95
0


Flow Rate: 0.3ml/min

Temp: 35ºC

Detection: Synapt G2Si QTOF