図4. フォーティス・バイオC4カラムとフォーティス・バイオC18カラムの比較

Samples: 1. Gly-Tyr (238.2MW)
2. Val-Tyr-Val (379.5MW)
3. Met-Enkephalin (573.7MW)
4. Leu-Enkephalin (555.6MW)
5. Angiotensin II (1046.2mW)
Columns:
FortisBIO C4, 300Å, 5µm, 50 x 2.1mm
FortisBIO C18, 300Å, 5µm, 50 x 2.1mm
Eluent A:
0.1% TFA in water
Eluent B:
0.1% TFA in ACN
Flow rate:
0.4mL/min
Gradient:
10-40% B in 10min
Detector: UV 220nm