Optimization of Fortis C8, 1.7µm for Nucleosides

Columns: Fortis C8, 1.7µm, 50 ~ 2.1mm (Fig. 1.)
Fortis C8, 1.7µm, 30 ~ 2.1mm (Fig. 2. and 3.)
Eluent: MeOH / H2O = 5 / 95
Flow rate: 0.3ml / min (Fig. 1. and 3.)
0.2ml / min (Fig. 2.)
Temp: 25ºC
Detector: UV254nm