| Column: | Fortis SpeedCore BIO C18, 300Å, 3.5µm, 150x4.6mm |
| Eluent A: | 0.1% formic acid in water |
| Eluent B: | 0.1% formic acid in IPA:ACN |
| Gradient: | 0-40 %B in 25mins 40-100 %B in 30mins |
| Flow rate: |
0.3ml/min |
| Temp: | 65ºC |
| Detector: | UV220nm |
| Peak 1: | Light Chain of IgG1 (25k Da) |
| Peak 2: | Impurity (only on Fortis SpeedCore BIO C18) |
| Peak 3: | Heavy Chain of IgG1(50k Da) |
| Peak 4: | Impurity |