フォーティス スピード・コア バイオ C18 カラムによる還元免疫グロブリン1 (Reduced IgG1 on Fortis SpeedCore BIO C18 Column)

 

Column: Fortis SpeedCore BIO C18, 300Å, 3.5µm, 150x4.6mm
Eluent A: 0.1% formic acid in water
Eluent B: 0.1% formic acid in IPA:ACN
Gradient: 0-40 %B in 25mins
40-100 %B in 30mins
Flow rate:
0.3ml/min
Temp: 65ºC
Detector: UV220nm
   
Peak 1: Light Chain of IgG1 (25k Da)
Peak 2: Impurity (only on Fortis SpeedCore BIO C18)
Peak 3: Heavy Chain of IgG1(50k Da)
Peak 4:

Impurity