PEGylation Positional Variants of Soluble TNF(Tumor Necrosis Factor) Receptor Type 1

(data courtesy Scott Buckel ; Amgen, Inc.)

Column: PolyCAT A 1000Å, 5µm, 200 × 4.6mm
Eluent A: 20mM sodium acetate, pH 5 and 20% ACN
Eluent B: 20mM sodium acetate, pH 5, 1M KCl and 20% ACN
Gradient: 1-16% B in 30 minutes
Flow rate:
 1.0ml / min
Detector: UV280nm