Columns: | 2 of PolySULFOETHYL A 300Å, 3µm, 100~4.6mm connected in series |
Sample: | Exenatide (refer to the above sequence) |
Eluent A: | 0.1M KH2PO4, pH 3.3 (adjusted after filtration and before addition of ACN) containing 43.6% ACN |
Eluent B: | Same thing but with 0.23M NaClO4 (adjust pH to 3.3 after dissolving the NaClO4 and KH2PO4 but before the addition of the ACN) |
Gradient: | 0% B in 0-5 minutes 0-60% B in 5-45 minutes 60-100% B in 45-50 minutes 100% B in 50-52 minutes 100-0% B in 52-54 minutes |
Flow rate: | 1.2ml / min |
Temp.: | 40ºC |
Detector: | UV214nm |