Analysis of Hemoglobin Variants (Quantitation of HbA1c)

Columns: PolyCAT A 1500Å, 3µm, 35~4.6mm
Sample: Hemoglobin variants
Eluent A: 20mM bis-tris + 2mM KCN, pH 6.9 (pH attained by blending 12mM bis-tris and 8mM bis-tris.HCl)
Eluent B: 20mM bis-tris + 2mM KCN + 200mM NaCl, pH 6.57 (pH attained by blending 7mM bis-tris and 13mM bis-tris.HCl)
Gradient: time (minutes)
0.00
0.10
1.65
2.15
2.40
2.90
3.10
4.00
% A
83
88
62
52
15
15
83
83
% B
17
12
38
48
85
85
17
17
Flow rate: 1.5ml / min
Detection: Absorbance at 410-415nm