| Columns: | PolyCAT A 1500Å, 3µm, 35×4.6mm | ||
| Sample: | Hemoglobin variants | ||
| Eluent A: | 20mM bis-tris + 2mM KCN, pH 6.9 (pH attained by blending 12mM bis-tris and 8mM bis-tris.HCl) | ||
| Eluent B: | 20mM bis-tris + 2mM KCN + 200mM NaCl, pH 6.57 (pH attained by blending 7mM bis-tris and 13mM bis-tris.HCl) | ||
| Gradient: | time (minutes) 0.00 0.10 1.65 2.15 2.40 2.90 3.10 4.00 |
% A 83 88 62 52 15 15 83 83 |
% B 17 12 38 48 85 85 17 17 |
| Flow rate: | 1.5ml / min | ||
| Detection: | Absorbance at 410-415nm | ||