Non-canonical Phosphorylation on PolySAX LP Column

Column: PolySAX LP, 300Å, 5µm, 200 x 4.6mm (204SX0503)
Gradient: Mobile Phase A: 20mM NaMePO4, pH=2.0, 70% ACN
Mobile Phase B: 300mM TEAP, pH=2.0, 10% ACN
(0f-5f, 100% A; 5f-47.9f, 100% B; 47.9f-52.9f, 100% B; 52.9f-53.9f; 100% A)
Flow: 1ml/min
Detection: 280nm